Composite

Part:BBa_K4949011:Design

Designed by: UManitoba_2023   Group: iGEM23_UManitoba   (2023-10-11)


lpp-OmpA-RPA1511 and sequences needed for gene expression


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 771
    Illegal XhoI site found at 487
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 597
    Illegal NgoMIV site found at 606
    Illegal NgoMIV site found at 832
    Illegal NgoMIV site found at 1006
    Illegal NgoMIV site found at 1066
    Illegal AgeI site found at 631
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 1309


Design Notes

This sequence has been codon optimized for expression in E.coli


Source

RPA1511 is a carboxyl esterase originally identified in Rhodopseudomonas palustris.

References